diff --git a/iseq.c b/iseq.c
index d1e567a17d..5349484ead 100644
--- a/iseq.c
+++ b/iseq.c
@@ -383,7 +383,6 @@ static rb_compile_option_t COMPILE_OPTION_DEFAULT = {
     OPT_OPERANDS_UNIFICATION, /* int operands_unification; */
     OPT_INSTRUCTIONS_UNIFICATION, /* int instructions_unification; */
     OPT_STACK_CACHING, /* int stack_caching; */
-    OPT_TRACE_INSTRUCTION, /* int trace_instruction */
     OPT_FROZEN_STRING_LITERAL,
     OPT_DEBUG_FROZEN_STRING_LITERAL,
     TRUE,			/* coverage_enabled */
@@ -410,7 +409,6 @@ set_compile_option_from_hash(rb_compile_option_t *option, VALUE opt)
     SET_COMPILE_OPTION(option, opt, operands_unification);
     SET_COMPILE_OPTION(option, opt, instructions_unification);
     SET_COMPILE_OPTION(option, opt, stack_caching);
-    SET_COMPILE_OPTION(option, opt, trace_instruction);
     SET_COMPILE_OPTION(option, opt, frozen_string_literal);
     SET_COMPILE_OPTION(option, opt, debug_frozen_string_literal);
     SET_COMPILE_OPTION(option, opt, coverage_enabled);
@@ -465,7 +463,6 @@ make_compile_option_value(rb_compile_option_t *option)
 	SET_COMPILE_OPTION(option, opt, operands_unification);
 	SET_COMPILE_OPTION(option, opt, instructions_unification);
 	SET_COMPILE_OPTION(option, opt, stack_caching);
-	SET_COMPILE_OPTION(option, opt, trace_instruction);
 	SET_COMPILE_OPTION(option, opt, frozen_string_literal);
 	SET_COMPILE_OPTION(option, opt, debug_frozen_string_literal);
 	SET_COMPILE_OPTION(option, opt, coverage_enabled);
@@ -953,7 +950,6 @@ iseqw_s_compile_file(int argc, VALUE *argv, VALUE self)
  *  * +:specialized_instruction+
  *  * +:stack_caching+
  *  * +:tailcall_optimization+
- *  * +:trace_instruction+
  *
  *  Additionally, +:debug_level+ can be set to an integer.
  *
diff --git a/iseq.h b/iseq.h
index 17066cb4bd..825d52bd84 100644
--- a/iseq.h
+++ b/iseq.h
@@ -203,7 +203,6 @@ struct rb_compile_option_struct {
     unsigned int operands_unification: 1;
     unsigned int instructions_unification: 1;
     unsigned int stack_caching: 1;
-    unsigned int trace_instruction: 1;
     unsigned int frozen_string_literal: 1;
     unsigned int debug_frozen_string_literal: 1;
     unsigned int coverage_enabled: 1;
diff --git a/vm_opts.h b/vm_opts.h
index 3fedf1d6e2..0d87e65654 100644
--- a/vm_opts.h
+++ b/vm_opts.h
@@ -18,7 +18,6 @@
  * Following definitions are default values.
  */
 
-#define OPT_TRACE_INSTRUCTION           1
 #define OPT_TAILCALL_OPTIMIZATION       0
 #define OPT_PEEPHOLE_OPTIMIZATION       1
 #define OPT_SPECIALISED_INSTRUCTION     1